Updating the rna polymerase ctd
Identification of CDK12-associating proteins reveals a strong enrichment for RNA-processing factors suggesting that CDK12 affects RNA processing events in two distinct ways: Indirectly through generating factor-binding phospho-epitopes on the CTD of elongating RNAPII and directly through binding to specific factors. Our study presents the first example of a nuclear factor requiring all three phospho-Ser marks within the heptad repeat of the CTD for high affinity binding and provides a molecular interpretation for the biochemical connection between the Ser(7) phosphorylation enrichment in the CTD of the transcribing RNA polymerase II over introns and co-transcriptional splicing events. “Specific interaction of the transcription elongation regulator TCERG1 with RNA polymerase II requires simultaneous phosphorylation at Ser2, Ser5, and Ser7 within the carboxyl-terminal domain repeat..” J Biol Chem, vol. For one well-known phospho CTD-associating protein, the histone methyltransferase Set2, we demonstrate a role in DNA damage resistance, and we show that this role requires the phospho CTD binding ability of Set2; surprisingly, Set2's role in damage resistance does not depend on its catalytic activity.




Using GST-CTD fusion protein substrates we find that CDK12/Cyclin K prefers a substrate with unmodified repeats or one that mimics prephosphorylation at the S7 position of the CTD; also the enzyme is sensitive to the inhibitor flavopiridol at higher concentrations. “Expression, purification, and identification of associated proteins of the full-length h CDK12/Cyclin K complex..” J Biol Chem, vol. Such a sequence-specific PCTD recognition is achieved through CTD-docking sites on FF4 and FF5 of TCERG1 but not FF6. In line with these results, extant data sets reveal a remarkable, highly significant overlap between phospho CTD-associating protein genes and DNA damage-resistance genes.
Co-crystallization of THZ531 with CDK12-cyclin K indicates that THZ531 irreversibly targets a cysteine located outside the kinase domain.
THZ531 causes a loss of gene expression with concurrent loss of elongating and hyperphosphorylated RNA polymerase II.
In particular, THZ531 substantially decreases the expression of DNA damage response genes and key super-enhancer-associated transcription factor genes.
Coincident with transcriptional perturbation, THZ531 dramatically induced apoptotic cell death.
Maybe it's the short profile text and single profile photo.
Find Out More None of the chatrooms require registration or sign up.
Shameka Scott was traveling to Atlanta when she heard commotion and turned to see the naked man running past security.
Now he has the problem of not having anybody interested in his lessons and teaching because the only way he can teach is through fear, so he will have to "scare them back to him".
Craigslist Texas Personals As the internet becomes more and more dominant in our lives, these dating sites are also getting more and more popular.
Rich Men Hookup helps you find that perfect partner through an immersive and effective online dating experience like no other.